CHROMOSOMAL MAPPING OF T-DNA INSERTS IN TRANSGENIC PETUNIA BY IN-SITUHYBRIDIZATION

The chromosomal location of T-DNA inserts in ten independently derived and confirmed transgenic plants of P. hybrida was detected by in situ hybridization. Nine transgenic plants had the T-DNA inserts at single sites distributed among each of the seven chromosomes; in one plant t he T-DNA inserts were detected on two different chromosomes. Although the T-DNA inserts were integrated randomly among the chromosomes, seve n of the 11 total inserts were located at or near the telomere. Thus, T-DNA inserts appear to have potential for tagging chromosomes and chr omosome fragments.