PBINPLUS - AN IMPROVED PLANT TRANSFORMATION VECTOR BASED ON PBIN19

Authors
VANENGELEN FA MOLTHOFF JW CONNER AJ NAP JP PEREIRA A STIEKEMA WJ
Citation
Fa. Vanengelen et al., PBINPLUS - AN IMPROVED PLANT TRANSFORMATION VECTOR BASED ON PBIN19, Transgenic research, 4(4), 1995, pp. 288-290
Citations number
6
Categorie Soggetti
Genetics & Heredity
Journal title
Transgenic research → ACNP
ISSN journal
09628819
Volume
4
Issue
4
Year of publication
1995
Pages
288 - 290
Database
ISI
SICI code
0962-8819(1995)4:4<288:P-AIPT>2.0.ZU;2-R
Abstract
We describe the construction of a new plant transformation vector, pBI NPLUS, based on the popular pBIN19 vector. Improvements over pBIN19 in clude location of the selectable marker gene at the left T-DNA border, a higher copy number in E. coli, and two rare restriction sites aroun d the multiple cloning site for easier cloning and analysis of T-DNA i nsertions in plant genomes.