P. Lundquist et al., AN IMPROVED METHOD FOR DETERMINATION OF THIOCYANATE IN PLASMA AND URINE, European journal of clinical chemistry and clinical biochemistry, 33(6), 1995, pp. 343-349
An improved spectrophotometric method is described for the determinati
on of thiocyanate in plasma and urine. Thiocyanate is adsorbed on a we
ak anion-exchange resin with strong affinity for chaotropic ions, and
eluted with perchlorate. Thiocyanate is then chlorinated by hypochlori
te and quantified according to the Konig (J Prakt Chem 1904; 69:105-37
) reaction by use of isonicotinic acid and 1,3-dimethyl-barbituric aci
d. The method affords a simple, rapid and sensitive assay for thiocyan
ate and has a detection limit of 0.93 mu mol/l. At thiocyanate concent
rations of 107.1 and 167.4 mu mol/l in plasma and urine the within-day
CVs were 0.69% and 1.1% respectively, and the total imprecision measu
red for a period of 65 days was 0.98%. Analytical recoveries were quan
titative both with urine and plasma samples.