STRUCTURAL AND SEROLOGICAL CHARACTERIZATION OF THE O-SPECIFIC POLYSACCHARIDE FROM LIPOPOLYSACCHARIDE OF ACINETOBACTER-CALCOACETICUS STRAIN-7 (DNA GROUP-1)

S-form lipopolysaccharide was isolated by phenol/water extraction from a strain of Acinetobacter calcoaceticus (DNA group 1). The structure of the O-antigenic polysaccharide was determined by compositional anal ysis and NMR spectroscopy of the de-O-acylated lipopolysaccharide. The isolated polysaccharide obtained after hydrolysis of lipopolysacchari de in 0.01 M trifluoroacetic acid has the following structure: [GRAPHI CS] in which Pyr is pyruvate. The O-acetyl substitution of D-Gal was n on-stoichiometric. The O-antigen was specifically recognised in wester n blots by polyclonal rabbit antisera.