USE OF A STOPPED-FLOW T-FORMAT SPECTROFLUOROMETER FOR SIMULTANEOUS KINETIC ANALYSES

The potential of the joint use of a T-format spectrofluorimeter and th e stopped-flow mixing technique in simultaneous kinetic determinations was studied. For this purpose, the direct resolution of a mixture of two analytes (neomycin and promethazine), by use of a condensation and an oxidation reaction that yield reaction products with similar maxim um excitation wavelengths but different emission wavelengths was chose n. The determination was readily accomplished in a few seconds by simu ltaneously measuring the respective initial rates in the corresponding emission channels. Mixtures of neomycin and promethazine in ratios be tween 8:1 and 1:9 were thus satisfactorily resolved. The relative stan dard deviation was 1.0-1.8%. The proposed method was applied to the an alysis of a commercially available pharmaceutical preparation, with a recovery of 99.3-102.0% for neomycin and 88.8-93.0% for promethazine.