A RAPID GENERAL-METHOD FOR THE IDENTIFICATION OF PCR PRODUCTS USING AFIBEROPTIC BIOSENSOR AND ITS APPLICATION TO THE DETECTION OF LISTERIA

Authors
STRACHAN NJC GRAY DI
Citation
Njc. Strachan et Di. Gray, A RAPID GENERAL-METHOD FOR THE IDENTIFICATION OF PCR PRODUCTS USING AFIBEROPTIC BIOSENSOR AND ITS APPLICATION TO THE DETECTION OF LISTERIA, Letters in applied microbiology, 21(1), 1995, pp. 5-9
Citations number
9
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Letters in applied microbiology → ACNP
ISSN journal
02668254
Volume
21
Issue
1
Year of publication
1995
Pages
5 - 9
Database
ISI
SICI code
0266-8254(1995)21:1<5:ARGFTI>2.0.ZU;2-N
Abstract
A 200-mer fragment of the flaA gene from Listeria monocytogenes was am plified using the polymerase chain reaction (PCR) incorporating biotin - and fluorescein amadite (FAM-)labelled primers. Methods are describe d for isolating the single stranded FAM-labelled 200-mer. A central po rtion of this 200-mer was successfully hybridized onto a complementary sequence coated onto a fibre optic biosensor. Advantages in the speci ficity and speed of this approach compared to standard agarose gel ele ctrophoresis and probing methods are discussed.