B. Hodrova et al., INTERACTION OF THE RUMEN FUNGUS ORPINOMYCES-JOYONII WITH MEGASPHAERA-ELSDENII AND EUBACTERIUM-LIMOSUM, Letters in applied microbiology, 21(1), 1995, pp. 34-37
The degradation and fermentation of microcrystalline cellulose were st
udied in monoculture of the polycentric anaerobic fungus Orpinomyces j
oyonii and in co-cultures with the rumen bacteria Megasphaera elsdenii
and Eubacterium limosum. More than 25% of cellulose hydrolysis produc
ts (glucose and cellodextrins) were released by the fungus into the me
dium after 8 d of cultivation. These products were metabolized by bact
eria in mixed cultures. In co-culture with the fungus M. elsdenii and
E. limosum increased the extent of microcrystalline cellulose degradat
ion by 10.12% and 7.96%, respectively. Biomass yield in co-cultures wa
s increased by 89.9% and 59.4% for M. elsdenii and E. limosum. Y-cellu
lose for fungus alone was 52.29 g dry matter mol(-1) glucose. These va
lues were 64.93 and 55.92 g mol(-1) glucose unit in co-culture with M.
elsdenii and E. limosum, respectively.