CHROMOSOME-14 ALTERATION IS ASSOCIATED WITH INCREASED COLLAGENASE EXPRESSION AND THE METASTATIC POTENTIAL OF MURINE MELANOMAS

The purpose of this study rr as to correlate abnormalities in chromoso me 14 with the invasive metastatic phenotype of K-1735 murine melanoma cells. Low metastatic K-1735 clone 10 and clone 23 cells were transfe cted with either basic fibroblast growth factor (bFGF), Kaposi's fibro blast grow th factor (kFGF), or c-H-ras gene. A high number of bFGF- a nd H-ras-transfected cells exhibited chromosome 14 rearrangements. The se cells also had increased expression of collagenase IV. The kFGF-tra nsfected cells were highly metastatic but did not have increased expre ssion of collagenase type IV, nor abnormalities in chromosome 14. The data imply that karyotypic changes in chromosome 14 are associated wit h increase expression of collagenase type IV. (C) Elsevier Science Inc ., 1996