METHODS AND REAGENTS - PURIFICATION OF HIS-TAG FUSION PROTEINS FROM ESCHERICHIA-COLI

Authors
HENGEN PN
Citation
Pn. Hengen, METHODS AND REAGENTS - PURIFICATION OF HIS-TAG FUSION PROTEINS FROM ESCHERICHIA-COLI, Trends in biochemical sciences, 20(7), 1995, pp. 285-286
Citations number
7
Categorie Soggetti
Biology
Journal title
Trends in biochemical sciences → ACNP
ISSN journal
09680004
Volume
20
Issue
7
Year of publication
1995
Pages
285 - 286
Database
ISI
SICI code
0968-0004(1995)20:7<285:MAR-PO>2.0.ZU;2-V
Abstract
Methods and reagents is a unique monthly column that highlights curren t discussions in the newsgroup bionet.molbio.methds-reagnts, available on the Internet. This month's column discusses a contaminating protei n from Escherichia coil found when histidine-tagged fusion proteins ar e purified by metal chelate affinity chromatography. For details on ho w to partake in the newsgroup, see the accompanying box.