COMPARISONS OF T-CELL RECEPTOR (TCR) V-BETA REPERTOIRES OF LAMINA PROPRIA AND PERIPHERAL-BLOOD LYMPHOCYTES WITH RESPECT TO FREQUENCY AND OLIGOCLONALITY

The TCR repertoires of CD4(+) and CD8(+) lamina propria and peripheral blood lymphocytes (PBL) were compared with respect to VP frequencies and oligoclonality. Disease-free colon specimens and paired peripheral blood samples were obtained from eight adult patients undergoing surg ical resections for colorectal carcinoma. Mononuclear cells were isola ted from the lamina propria and peripheral blood and separated into CD 4(+) and CD8(+) cells by immunomagnetic adsorbtion. Analysis of VP fre quencies by qPCR revealed that PBL and lamina propria lymphocytes (LPL ) from the same individual have very different repertoires, especially within the CD8(+) population. Furthermore, CD8(+), but not CD4(+), LP L display extensive oligoclonality, similar to that which has previous ly been reported for CD8(+) PBL. However, the oligoclonal receptors ob served in CD8(+) LPL are, in general, distinct from those observed in CD8(+) PBL, and differ for each individual. These observations indicat e that the TCR repertoires of LPL are as diverse as PBL, and suggest t hat LPL and PBL are normally exposed to different sets of antigens. In addition, these observations provide a baseline for examining the eff ects of various disease states and environmental insults on the LPL re pertoire. (C) 1995 Academic Press, Inc.