Two types of carbon paste electrodes containing ubiquinone (CoQ) and g
lucose oxidase (GOD) were constructed, and their electrochemistry and
amperometric response to substrate were investigated. For one type of
electrode in which the surface of carbon particles was covered with a
part of CoQ molecules added, it was found that the CoQ adsorbed on the
carbon particles exerted an insulating effect on the electrode reacti
on. For the other type of electrode in which the surface was preadsorb
ed with GOD and most of CoQ was dissolved in the liquid paraffin phase
, it was demonstrated that CoQ served as an electron transfer mediator
. The latter electrode showed amperometric responses to glucose in the
applied potential range 300 - 500 mV and a linear response in the ran
ge 0.2 - 4.0 mM in an electrolyte solution (pH 7.0).