CATALYTIC-OXIDATION OF NADH AT A METHYLENE-GREEN CHEMICALLY MODIFIED ELECTRODE AND FIA APPLICATIONS

Chemically modified electrodes with Methylene Green (MG) incorporated into carbon paste exhibit excellent electrocatalytic ability for reduc ed nicotinamide adenine dinucleotide (NADH), reducing oxidation overpo tential by 600mV compared with the direct oxidation of NADH at unmodif ied electrode. In flow injection analysis (FIA) of NADH, MG-modified c arbon paste electrodes show high catalytic activity and stability, wit h linear response range over about 3 orders of magnitude and a detecti on limit of 1.0 pmol. Furthermore, the influence of various experiment al conditions was explored for optimum analytical performance.