SIMULTANEOUS DETERMINATION OF TINIDAZOLE AND FURAZOLIDONE IN SUSPENSION BY HPTLC AND HPLC

High performance thin layer chromatographic (HPTLC) and high performan ce liquid chromatographic (HPLC) methods were developed for the simult aneous determination of Tinidazole and Furazolidone in suspension. In the HPTLC method the separation of Tinidazole and Furazolidone was car ried out on silica gel 60F(254) HPTLC glass plate using chloroform: me thanol: ammonia (9:1:0.1 v/v) as a mobile phase. Rf values obtained we re 0.63 and 0.79 for Furazolidone and Tinidazole respectively. Densito metric evaluation was done at 335 nm. Linearity was obtained within th e concentration range 10-50 mu g/ml and 3.5-17.5 mu g/ml for Tinidazol e and Furazolidone respectively. The second method is based on high pe rformance liquid chromatography on a reversed phase column (mu Bondapa k C-18) using a mobile phase comprised of water : acetonitrile : triet hylamine (80:20:0.1 v/v) adjusted to pH = 3.0 with dil. phosphoric aci d. Retention times were 5.24 and 7.82 min for Tinidazole and Furazolid one respectively at a flow rate of 1.5 ml/min. Detection was done at 3 35 nm. Linearity was obtained within the concentration range 30-180 mu g/ml and 10.5-63 mu g/ml for TinidazoIe and Furazolidone resp.