PLATELET-ADHESION TO CULTURED BOVINE CEREBRAL MICROVASCULAR ENDOTHELIAL-CELLS BY STIMULATION OF PLATELET-ACTIVATING-FACTOR AND ANTAGONISM OF DRUGS

AIM: To study platelet activating factor (PAF) stimulating the platele ts to adhere to cultured bovine cerebral microvascular endothelial cel ls (CMEC) and the inhibitory effect of triazelodiazepine (WEB), 1,5-bi s-(3, 4-dimethoxyphenyl)-tetrahydro-(4H)-pyran (DMPP), tetrandrine (Te t). METHODS: The platelets adhesion to CMEC and the inhibitory effect of drugs were investigated by [H-3]adenine labeling of rabbit blood pl atelet. RESULTS: The platelet adhesion to CMEC was increased by 36 % v s control after CMEC was stimulated with PAF 10 nmol L(-1) for 25 min. WEB 0.1, 1, 10 mmol L(-1) or DMPP 0.1, 1, 10 mmol L(-1) or Tet 0.1, 1 , 10 mmol L(-1) inhibited the PAF stimulating platelet adhesion to CME C by 5.4 %, 16.3 %, 20.1 %; 13.7 %, 19.4 %, 22.4 %; and 5.5 %, 23.1 %, 32.6 %, respectively. CONCLUSION: DMPP and Tet inhibited the PAF acti on in cerebral vascular system.