SOMATIC AND GERMINAL ACTIVITIES OF MAIZE ACTIVATOR (AC) TRANSPOSASE MUTANTS IN TRANSGENIC TOBACCO

Citation
R. Kunze et al., SOMATIC AND GERMINAL ACTIVITIES OF MAIZE ACTIVATOR (AC) TRANSPOSASE MUTANTS IN TRANSGENIC TOBACCO, Plant journal, 8(1), 1995, pp. 45-54
Citations number
35
Categorie Soggetti
Plant Sciences",Biology
Journal title
ISSN journal
09607412
Volume
8
Issue
1
Year of publication
1995
Pages
45 - 54
Database
ISI
SICI code
0960-7412(1995)8:1<45:SAGAOM>2.0.ZU;2-4
Abstract
The properties have been investigated of two deletion derivatives of t he transposase protein (TPase) of maize transposable element Ac in tra nsgenic tobacco. The wildtype and mutant TPases were expressed as fusi ons to the cauliflower mosaic virus 35S promoter. A deletion of 102 am ino acids from the N-terminus, TPase(103-807), induces Ds excisions fr om a SPT::Ds reporter locus with a higher frequency than the wild-type TPase. The increased transpositional activity of TPase(103-807) is a dominant trait, as seedlings coexpressing truncated and wild-type TPas e show the characteristic TPase(103-807) variegation phenotype. A tran spositionally inactive TPase deletion derivative lacking 188 amino aci ds from the N-terminus inhibits the transpositional activity of the wi ld-type TPase.