CAPILLARY ELECTROPHORESIS OF ISOQUINOLINE ALKALOIDS FROM CHELIDONIUM-MAJUS L

Citation
Pg. Pietta et al., CAPILLARY ELECTROPHORESIS OF ISOQUINOLINE ALKALOIDS FROM CHELIDONIUM-MAJUS L, Phytochemical analysis, 6(4), 1995, pp. 196-202
Citations number
18
Categorie Soggetti
Biology,"Chemistry Analytical","Plant Sciences
Journal title
ISSN journal
09580344
Volume
6
Issue
4
Year of publication
1995
Pages
196 - 202
Database
ISI
SICI code
0958-0344(1995)6:4<196:CEOIAF>2.0.ZU;2-V
Abstract
The determination of six different isoquinoline alkaloids from Chelido nium majus (leaves and roots) using capillary electrophoresis is descr ibed. Analyses were performed on fused silica capillaries with 66 mM p hosphate buffer at pH 2.4 containing 10% methanol. The analytes were m onitored by on-line ultraviolet (UV) detection to identify sanguinarin e, chelerythrine, berberine and chelidonine. Both coptisine and DL-sty lopine were isolated by semi-preparative high performance liquid chrom atography (HPLC), and their structure assessed by UV and mass spectrom etry. Capillary electrophoresis is confirmed as a useful complementary technique to HPLC.