G. Almahbobi, ADHESION OF INTERMEDIATE FILAMENTS AND LIPID DROPLETS IN ADRENAL-CELLS STUDIED BY FIELD-EMISSION SCANNING ELECTRON-MICROSCOPY, Cell and tissue research, 281(2), 1995, pp. 387-390
High-resolution field emission scanning electron microscopy was used t
o study the organisation of intermediate filaments around lipid drople
ts and their binding to these droplets, in primary culture of bovine a
drenal cells. Whole-mount preparations of intermediate filaments and b
ound lipid droplets were prepared from cells grown on Formvar-coated g
rids and processed by freeze-drying. Intermediate filaments were seen
as an interconnected network enveloping the entire droplet. The bound
filaments appear to be directly adherent to the surface of the droplet
and hence take on its curved contour. The binding of the filaments to
the droplets was determined by means of tilting. This study provides
a new approach to investigate the cytoskeleton and its associated stru
ctures with high-resolution three-dimensional images.