PROPERTIES OF DORMANT CELLS IN STATIONARY-PHASE CULTURES OF MICROCOCCUS-LUTEUS DURING PROLONGED INCUBATION

Citation
Gv. Mukamolova et al., PROPERTIES OF DORMANT CELLS IN STATIONARY-PHASE CULTURES OF MICROCOCCUS-LUTEUS DURING PROLONGED INCUBATION, Microbiology, 64(3), 1995, pp. 284-288
Citations number
20
Categorie Soggetti
Microbiology
Journal title
ISSN journal
00262617
Volume
64
Issue
3
Year of publication
1995
Pages
284 - 288
Database
ISI
SICI code
0026-2617(1995)64:3<284:PODCIS>2.0.ZU;2-1
Abstract
A previously discovered phenomenon of dormant cell formation by Microc occus luteus cultures [9] during prolonged (for 3 to 9 months) station ary phase was studied. Over this period, there was a decrease in the n umber of cells capable of forming colonies on solid medium, the total cell count remaining constant. The rate and the magnitude of this decr ease depended on cell concentration observed at the beginning of the s tationary phase and also on the composition of the incubation medium. Dormant cells were characterized by almost complete lack of endogenous respiration, a 30 - 50% decrease in cytochrome content, change in pho spholipid composition, and also deteriorated permeability barrier of t he cytoplasmic membrane. It was found that the ability of dormant cell s to resuscitate and turn into normal culturable forms correlates with the restoration of the permeability barrier and with the lysophosphol ipid content in membranes.