Propylene glycol (PG) is a gluconeogenic precursor widely used to prev
ent and treat ketosis postpartum. The study has investigated the effec
ts of PG administration to dairy cows at mid stage of lactation. Accor
ding to a 3 x 3 latin square design, three Italian Brown lactating cow
s (125 +/- 7 days in milk) fitted with rumen cannula were fed a corn s
ilage based diet (CP 14.7%, NDF 41.1% DM) with 0, 200 or 400 g day(-1)
PG added. Dry matter intake was increased by feeding PG with a signif
icant quadratic component per dose (16.2, 17.2 and 16.5 kg day(-1) for
0, 200 and 400 g PG day(-1), respectively). Milk yield was not affect
ed by PG, averaging 17.1 kg day(-1). Average daily gain increased from
64 to 206 and 302 g day(-1) when cows received 200 and 400 g day(-1)
of PG (linear component per dose P < 0.05). Digestibility of the diet
did not differ among treatments, whereas repeated rumen fluid samples,
taken 0, 2, 4, 6 and 8 h after the meal, showed a consistently lower
acetate to propionate ratio when feeding PG. Blood insulin was not aff
ected by PG administration. Except for therapeutic treatments, PG admi
nistration to dairy cows at mid stage of lactation should be advised a
gainst. Despite the positive effect on intake, administration of the a
dditive, increasing the molar percentage of rumen propionate, contribu
tes to shift the energy partition from milk production to liveweight g
ain.