EFFECTS OF TEMPERATURE AND STORAGE TIME ON PIN PULL-OUT TESTING IN HARVESTED CANINE FEMURS

Effects of temperature and storage time on canine bone-transfixation p in specimens were tested by comparing pin pull-out forces. A total of 16 femurs from 8 mature dogs were tested. Five nonthreaded Steinmann p ins were placed through both cortices in the diaphysis of each femur. The femurs were then sectioned tranversely between each pin, with a bo ne-pin specimen placed evenly into each of 5 groups prior to biomechan ical testing. Four bone-pin specimen groups were stored at -20 or -70 C for 14 or 28 days, while 1 specimen group was immediately tested. Pu ll-out forces for frozen groups were compared with pull-out forces for the fresh group. Using two-way ANOVA, there was no statistical differ ence in mean axial-extraction forces among bone-pin specimens in any o f the tested groups. It is concluded that acute pin pull-out forces ar e not significantly affected by freezing temperature or time. However, specimens stored at -20 C for as few as 14 days had a trend for incre ased pull-out forces, compared with freshly harvested specimens. There fore, the authors recommend storage of bone-pin specimens at -70 C whe n possible.