THE USE OF THE STRS HUMTHO1, HUMVWA31 A, HUMF13A1, HUMFES/FPS, HUMLPLIN FORENSIC APPLICATION - VALIDATION STUDIES AND POPULATION-DATA FOR GALICIA (NW SPAIN)/

The 5 tetranucleotide short tandem repeats, HUMTH01, HUMVWA31/A, HUMF1 3A1, HUMFES/FPS and HUMLPL were studied using different electrophoreti c methods and PCR amplification conditions in order to optimize the ty ping conditions. A genetic population study in the population of Galic ia was carried out and the allele and genotype frequencies are given. Compliance with the Hardy-Weinberg equilibrium was tested using differ ent statistical parameters, with clear advantages resulting in favor o f using the exact test (Guo-Thompson method) instead of conventional c hi-square methods. Some statistical parameters of forensic interest (P D, CE, h) were also calculated. There were no mutations found in a tot al of 73 paternal meioses and 101 maternal meioses. Abnormal electroph oretic mobility was found in the AT-rich STR HUMF13A1 under non-denatu ring conditions and, therefore, the use of denaturing conditions is ab solutely necessary. No ''stutter'' bands were found, although double p eaks in the HUMFES/FPS system were observed in some samples. The advan tage of using automated sequencers with fluorescent technology is also reported.