A nested PCR approach has been developed especially for the detection
of small amounts of cytomegalovirus (CMV) DNA in autopsy samples. Lung
tissue and submandibular glands in 118 cases of infant death (92 SIDS
cases, 13 natural deaths due to other defined causes and 13 unnatural
deaths) were investigated by this technique and compared to the resul
ts obtained by other CMV detection methods (histology, immunohistochem
istry, in situ hybridization and PCR). CMV-DNA could be detected in th
e lung tissue in 7 cases of SIDS using nested PCR. Compared to convent
ional PCR (3 positive cases in lung tissue) the nested approach always
gave glear results and showed less additional bands. In all cases whe
re CMV could be detected in the lungs, positive results were also obta
ined in the submandibular glands. The nested PCR method proved to be a
more sensitive technique than the other detection methods including P
CR and hot start, and even minimal amounts of target DNA could be dete
cted in the presence of human and bacterial background DNA.