EFFECT OF LOCAL CHOLECYSTOKININ-8 ADMINISTRATION ON EXTRACELLULAR LEVELS OF AMINO-ACIDS AND GLYCOLYTIC PRODUCTS MONITORED BY IN-VIVO MICRODIALYSIS IN THE FRONTOPARIETAL CORTEX OF THE RAT
O. Godukhin et al., EFFECT OF LOCAL CHOLECYSTOKININ-8 ADMINISTRATION ON EXTRACELLULAR LEVELS OF AMINO-ACIDS AND GLYCOLYTIC PRODUCTS MONITORED BY IN-VIVO MICRODIALYSIS IN THE FRONTOPARIETAL CORTEX OF THE RAT, Neuroscience letters, 194(1-2), 1995, pp. 29-32
The effects of local cholecystokinin-8 (CCK-8) administration on corti
cal extracellular levels of amino acids, catecholamines and metabolism
products were studied in the halothane anaesthetized rat by in vivo m
icrodialysis. CCK-8 (10 mu M), administrated via a microdialysis probe
, produced a significant increase in the levels of aspartate, glutamat
e and gamma-aminobutyric acid (GABA), but not of 3,4-dihydroxyphenylac
etic acid (DOPAC), homovanillic acid (HVA), lactate and pyruvate, whic
h were simultaneously monitored. The increase in aspartate and glutama
te levels produced by CCK-8 was about 3-4-fold. The effect of CCK-8 on
aspartate levels was significantly inhibited by the CCKB antagonist,
L-365,260 (20 mg kg(-1), s.c.), but not by the CCKA antagonist, L-364,
718 (20 mg kg(-1) s.c.). In contrast, the increase in glutamate levels
was inhibited by both L365,260 and L364,718. GABA was slightly, but s
ignificantly increased (similar to 30%), by local CCK-8 and was inhibi
ted by both CCK antagonists. The present results show that CCK-8 exert
s a strong modulatory action on both aspartate and glutamate release i
n rat cortex. While the effect of CCK-8 on aspartate is selectively me
diated via CCKB receptor subtype, the effect of CCK-8 on glutamate app
ears to be mediated via both CCKA and CCKB receptor subtypes.