FURTHER STUDY ON THE CHIRAL SEPARATION POWER OF A SOLUBLE NEUTRAL BETA-CYCLODEXTRIN POLYMER

Enantiomeric separation of some basic compounds, namely selegiline, am phetamine, and clenbuterol, was studied by capillary electrophoresis u sing an uncharged beta-cyclodextrin polymer added to the background el ectrolyte at pH 2.5. Both complexation and resolution were influenced by the concentration of the chiral polymer confirming our previous res ults obtained in our earlier work for a wide number of pharmaceutical compounds. In this further study, we examined the influence of differe nt organic additives to the background electrolyte on the enantioselec tivity of the chiral selector, also using an extended number of analyt es. In most cases, the use of an organic additive resulted in a decrea se of resolution, However opposite to that, in some cases, e.g. ephedr ine, the organic solvent proved to be essential to achieve enantiomeri c resolution, Furthermore the influence of the capillary temperature o n the resolution of the analytes was evaluated, Increase of temperatur e had a deleterious effect on the resolution of the enantiomers. For e phedrine, however, relatively high temperature (50 degrees C) proved t o be advantageous, for the resolution of the optical isomers.