MURINE EPIDERMAL V-GAMMA-5 V-DELTA-1-T-CELL RECEPTOR(+) T-CELLS RESPOND TO B-CELL LINES AND LIPOPOLYSACCHARIDES/

The V gamma 5/V delta 1(+)-T-cell receptor (TCR)-bearing T-cell clone, 2CBET-3, was generated from C57BL/6 mice., Upon stimulation, 2CBET-3 cells produce interleukin (IL)-3, granulocute-macrophage colony-stimul ating factor, and tumor necrosis factor-alpha, but not IL-2, IL-4, IL- 5, IL-6, IL-8, IL-10, macrophage colony-stimulating factor, or interfe ron-gamma., These cells were evaluated for their ability to be stimula ted by a variety of murine cell lines, including fibroblasts, trophobl asts, melanoma cells, embryonic carcinomas, B-cell lymphomas, mastocyt oma cells, and keratinocytes. The human B-lymphoma cell line, Daudi, a lso was included in these studies. We found,that 2CBET-3 cells produce d cytokines up to several hundredfold above the control levels in resp onse to the B-cell lines, Daudi, and A20/2J, but not to the B-cell lin e 439.4.2. After fixation with glutaraldehyde, Daudi and A20/2J contin ued to stimulate this gamma delta T-cell line. 2CBET-3 cells also resp onded to the keratinocyte line PAM212, but not to another, XB-2. When lipopolysaccharides (LPS) from Escherichia call or S. typhimurium were added to 2CBET-3 cells in the presence of A20/2J cells, 2CBET-3 cells responded with increased cytokine production compared with the cytoki ne production in the presence of A20/2J cells alone. 2CBET-3 cells by themselves did not respond to LPS alone or to supernatants from A20/2J cells incubated with LPS, Unlike 2CBET-3, the epidermal T-cell hybrid oma 70BET-49, expressing a V gamma 5/V delta 1-TCR identical to that o f 2CBET-3, did not respond to A20/2J cells in the presence or absence of LPS, suggesting a requirement for molecules other than the TCR for V gamma 5/V delta 1-TCR(+) T-cell stimulation by the B-cell lines and by LPS, This unique reactivity of gamma delta-TCR(+) cells is differen t from that of alpha beta-TCR(+) cells and map reflect a functional sp ecialization of gamma delta-TCR(+) cells in the response to bacterial infections.