ASSIGNMENT OF THE BACKBONE CARBONYL RESONANCES IN N-15-LABELED PROTEINS WITH C-13 AT NATURAL-ABUNDANCE BY A 2D TRIPLE-RESONANCE CORRELATIONTECHNIQUE

Authors
KRISTENSEN SM SORENSEN MD LED JJ
Citation
Sm. Kristensen et al., ASSIGNMENT OF THE BACKBONE CARBONYL RESONANCES IN N-15-LABELED PROTEINS WITH C-13 AT NATURAL-ABUNDANCE BY A 2D TRIPLE-RESONANCE CORRELATIONTECHNIQUE, Journal of biomolecular NMR, 5(4), 1995, pp. 411-414
Citations number
18
Categorie Soggetti
Biology,Spectroscopy
Journal title
Journal of biomolecular NMR → ACNP
ISSN journal
09252738
Volume
5
Issue
4
Year of publication
1995
Pages
411 - 414
Database
ISI
SICI code
0925-2738(1995)5:4<411:AOTBCR>2.0.ZU;2-W
Abstract
A 2D NMR experiment for assignment of backbone carbon resonances in sm all and medium-sized N-15-labelled proteins with C-13 at natural abund ance is presented. The experiment is a two-dimensional variant of the HNCO triple-resonance experiment and is demonstrated by application to a 6 kDa protein at relatively low concentration (2 mM) and temperatur e (30 degrees C). The experiment is particularly suitable for assignme nt of carbonyl resonances.