SELECTIVE EXCITATION OF INTENSE SOLVENT SIGNALS IN THE PRESENCE OF RADIATION DAMPING

Selective water excitation schemes are provided which rely on the radi ation damping effect in probeheads characterized by high quality facto rs. The schemes are implemented in homonuclear NOE and ROE experiments , designed for the selective observation of water-protein cross peaks and their assignment using standard probeheads. The one-dimensional NO E and ROE experiments selectively record the cross section through the water signal usually measured in two-dimensional NOESY and ROESY spec tra, and the two-dimensional NOE-NOESY and ROE-NOESY experiments selec tively measure the cross section through the water line from 3D NOESY- NOESY and ROESY-NOESY spectra, respectively.