G. Otting et E. Liepinsh, SELECTIVE EXCITATION OF INTENSE SOLVENT SIGNALS IN THE PRESENCE OF RADIATION DAMPING, Journal of biomolecular NMR, 5(4), 1995, pp. 420-426
Selective water excitation schemes are provided which rely on the radi
ation damping effect in probeheads characterized by high quality facto
rs. The schemes are implemented in homonuclear NOE and ROE experiments
, designed for the selective observation of water-protein cross peaks
and their assignment using standard probeheads. The one-dimensional NO
E and ROE experiments selectively record the cross section through the
water signal usually measured in two-dimensional NOESY and ROESY spec
tra, and the two-dimensional NOE-NOESY and ROE-NOESY experiments selec
tively measure the cross section through the water line from 3D NOESY-
NOESY and ROESY-NOESY spectra, respectively.