S. Salomaa et al., UNSTABLE AND STABLE CHROMOSOMAL-ABERRATIONS IN LYMPHOCYTES OF PEOPLE EXPOSED TO CHERNOBYL FALLOUT IN BRYANSK, RUSSIA, International journal of radiation biology, 71(1), 1997, pp. 51-59
Citations number
40
Categorie Soggetti
Radiology,Nuclear Medicine & Medical Imaging","Nuclear Sciences & Tecnology
Analyses of unstable and stable chromosomal aberrations in peripheral
blood lymphocytes were used in the assessment of radiation exposure of
residents of a village situated in the Chernobyl fallout-contaminatio
n zone of Bryansk, Russia. Blood samples were taken from subjects resi
ding in villages with high (>1100 kBq/m(2) Cs-137; Mirnyi) and very lo
w (<37 kBq/m(2) Cs-137; Krasnyi Rog) contamination, 7 years after the
Chernobyl accident. The groups were matched by age, sex, smoking habit
s and previous medical radiological exposures. A total of 200 people (
100 exposed, 100 controls) were analysed for the presence of unstable
aberrations from Giemsa-stained slides. To study stable aberrations, c
hromosome painting analyses were performed on 100 subjects (50 exposed
, 50 controls), using painting probes for chromosomes 1, 2 and 4 and a
pancentromeric probe. People living in the contaminated area showed s
ignificantly higher rates of unstable chromosome-type aberrations but
not chromatid-type aberrations in their lymphocytes, indicating radiat
ion exposure as a causative factor for the observed difference. No sig
nificant differences were found in the aberration rates between the tw
o areas by the chromosome painting method. The levels of chromosome ex
changes were low in both populations, but consistently higher in Mirny
i compared with the control area. The magnitude of radiation exposure
resulting from Chernobyl fallout was estimated on the basis of excess
stable chromosomal aberrations in the lymphocytes of the Mirnyi popula
tion compared with the controls.