HETEROGENEITY OF VP4 NEUTRALIZATION EPITOPES AMONG SEROTYPE P1A HUMANROTAVIRUS STRAINS

Citation
Jf. Contreras et al., HETEROGENEITY OF VP4 NEUTRALIZATION EPITOPES AMONG SEROTYPE P1A HUMANROTAVIRUS STRAINS, Clinical and diagnostic laboratory immunology, 2(4), 1995, pp. 506-508
Citations number
30
Categorie Soggetti
Immunology,"Infectious Diseases","Medical Laboratory Technology",Microbiology
ISSN journal
1071412X
Volume
2
Issue
4
Year of publication
1995
Pages
506 - 508
Database
ISI
SICI code
1071-412X(1995)2:4<506:HOVNEA>2.0.ZU;2-A
Abstract
We have used serotype-specific VP4 and VP7 neutralizing monoclonal ant ibodies (Nt-MAbs), as well as subgroup (SG)-specific MAbs, to characte rize by enzyme immunoassay rotavirus strains isolated from diarrheic i nfants in the city of Monterrey, Mexico, from July 1993 to March 1994. Of a total of 465 children studied, 140 were rotavirus positive, incl uding 3 patients infected with non-group A rotaviruses. The SG and VP7 (G) serotype specificities could be determined for 118 (84%) of the 1 40 rotavirus-positive stool specimens; 4 rotavirus strains were seroty pe G1 and SGII; 1 strain was serotype G2 and SGI+II; 112 strains were serotype G3 and SGII; 1 strain was serotype G3 and SGI; and none of th e strains was serotype G4. Fifty-eight specimens, representing the 13 different group A rotavirus electropherotypes detected, were chosen fo r VP4 (P) serotyping. Of these, 48 (83%) strains reacted with the P1A serotype-specific Nt-MAb 1A10. None of the strains reacted with the se rotype P2-specific Nt-MAbs tested. Not all viruses that reacted with N t-MAb 1A10 were recognized by Nt-MAbs 2A3 and 2G1, which also recogniz e P1A strains, indicating heterogeneity of neutralization epitopes amo ng serotype P1A human rotaviruses. This heterogeneity could be relevan t for the specificity of the VP4-mediated neutralizing antibody immune response and indicates the need for antigenic characterization, in ad dition to genomic typing, of the VP4 proteins of circulating human rot avirus field strains.