EMBRYO CULTURE IN EXPLANTED OVIDUCTS IN MICE AND CATTLE

Developmental block of early embryos is due to the lack of some compon ents in the culture medium and/or caused by inappropriate environmenta l conditions for embryonic development. It was shown in our experiment s that the development of mouse and bovine embryos under the influence of oviducts in vitro can provide us with a model to analyze the ovidu ctal factors that promote embryonic development. In the first experime nt, it was shown that mouse zygotes developed to the blastocyst stage when a coculture system with ampulla was applied, and the duration of embryo coculture significantly affected the further development of emb ryos. In the second experiment, it was shown that the proportion of bl astocysts of cleaved ova obtained after coculture with mouse ampulla w as significantly higher than that obtained after coculture with a gran ulosa cell monolayer or culture without cells.