HORMONAL-REGULATION IN THE PRODUCTION OF MACROPHAGE-COLONY-STIMULATING FACTOR AND TRANSFORMING GROWTH-FACTOR-BETA BY HUMAN ENDOMETRIAL STROMAL CELLS IN CULTURE
H. Kanzaki et al., HORMONAL-REGULATION IN THE PRODUCTION OF MACROPHAGE-COLONY-STIMULATING FACTOR AND TRANSFORMING GROWTH-FACTOR-BETA BY HUMAN ENDOMETRIAL STROMAL CELLS IN CULTURE, Hormone research, 44, 1995, pp. 30-35
The effects of gonadal steroids on the secretion and gene expression o
f macrophage colony-stimulating factor (M-CSF) and on the secretion of
transforming growth factor (TGF)-beta 1 and TGF-beta 2 by human endom
etrial stromal cells (ESCs) were examined by an in vitro system of ESC
differentiation (decidualization). M-CSF production by ESCs was dose-
dependently enhanced by the addition of progesterone or testosterone,
while estradiol treatment had no effect. TGF-beta 2 secretion by ESCs
was inhibited by progesterone, estradiol and testosterone treatment, a
nd on the contrary, slight enhancement by estradiol was observed in TG
F-beta 1 secretion. These findings indicate that human ESCs produce cy
tokines of M-CSF and TGF-beta s, which are important for the growth an
d differentiation of the peri-implantation embryo as well as local imm
une cells under direct control of gonadal steroidal actions, and sugge
st a novel network between endocrine and immune systems in the human e
ndometrium.