SELECTIVITY OF REVERSE TRANSCRIPTASES .1. SUBSTRATE PROPERTIES OF NOVEL 2',3'-UNSATURATED ACYCLIC NUCLEOTIDE ANALOGS

A series of new acyclic nucleotide analogs, (Z)-pyrophosphoryl(phospho nyloxymethyl)but-2-enyl derivatives of purines and pyrimidines, was sy nthesized. Their substrate and inhibitory properties toward some rever se transcriptases and DNA polymerases were evaluated. The compounds sy nthesized were found to be substrate inhibitors of human immunodeficie ncy virus and avian myeloblastosis virus reverse transcriptases. A num ber of conclusions was made on the relationship between the structure and substrate properties for nucleotide analogs and the substrate spec ificity of reverse transcriptases.