3-DIMENSIONAL CRYSTALS OF CYTOCHROME-C-OXIDASE FROM THERMUS-THERMOPHILUS DIFFRACTING TO 3.8 ANGSTROM RESOLUTION

Authors
SOULIMANE T GOHLKE U HUBER R BUSE G
Citation
T. Soulimane et al., 3-DIMENSIONAL CRYSTALS OF CYTOCHROME-C-OXIDASE FROM THERMUS-THERMOPHILUS DIFFRACTING TO 3.8 ANGSTROM RESOLUTION, FEBS letters, 368(1), 1995, pp. 132-134
Citations number
25
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
368
Issue
1
Year of publication
1995
Pages
132 - 134
Database
ISI
SICI code
0014-5793(1995)368:1<132:3COCFT>2.0.ZU;2-P
Abstract
The ba(3)-type cytochrome-c oxidase from Thermus thermophilus has been crystallized in its native form. Crystallization was achieved by the batch and the vapour diffusion sitting drop methods using polyethylene glycol monomethyl ether 2000 as precipitating agent in the presence o f octyl-beta-D-thioglucoside as detergent. The crystals diffract to 3. 8 Angstrom, belong to the space group P2 or P2(1) and have unit cell. dimensions of a = 80.7 Angstrom; b = 116.0 Angstrom; c = 156.9 Angstro m and beta = 104.4 degrees, The asymmetric unit contains true ba(3)-ty pe oxidase molecules.