Jy. Chatton et al., SIMULTANEOUS MEASUREMENTS OF CA2-INDUCED CA2+ OSCILLATIONS( IN THE INTRACELLULAR STORES AND THE CYTOSOL OF HEPATOCYTES DURING HORMONE), FEBS letters, 368(1), 1995, pp. 165-168
Simultaneous Ca2+ measurements in the cytosol and intracellular stores
(IS) of rat hepatocytes were performed using two Ca2+-sensitive probe
s (Fluo-3 and Mag-fura-2), and combined whole-cell patch clamp and flu
orescence microscopy. A steady-state Ca2+ concentration of similar to
630 mu M was estimated in the IS. alpha(1)-Adrenergic stimulation indu
ced periodic elevations of cytosolic Ca2+ and parallel synchronized tr
ansient declines in the IS. Subsequent application of the intracellula
r Ca2+-pump inhibitor thapsigargin resulted in a release of Ca2+ from
the IS to reach a level of Ca2+ depletion much lower than the lowest t
ransient decline observed during the oscillations.