A NEW METHOD FOR THE TIME-RESOLVED MEASUREMENT OF PHOSPHATE RELEASE IN PERMEABILIZED MUSCLE-FIBERS

Authors
FERENCZI MA HE ZH CHILLINGWORTH RK BRUNE M CORRIE JET TRENTHAM DR WEBB MR
Citation
Ma. Ferenczi et al., A NEW METHOD FOR THE TIME-RESOLVED MEASUREMENT OF PHOSPHATE RELEASE IN PERMEABILIZED MUSCLE-FIBERS, Biophysical journal, 68(4), 1995, pp. 191-194
Citations number
2
Categorie Soggetti
Biophysics
Journal title
Biophysical journal → ACNP
ISSN journal
00063495
Volume
68
Issue
4
Year of publication
1995
Supplement
S
Pages
191 - 194
Database
ISI
SICI code
0006-3495(1995)68:4<191:ANMFTT>2.0.ZU;2-#
Abstract
A new method for the measurement of phosphate release in contracting a nd relaxed permeabilized muscle fibers is described. The assay is base d on a genetically engineered phosphate-binding protein labeled with a coumarin fluorescent probe, which binds inorganic phosphate tightly a nd shows a fourfold increase in fluorescence upon binding. Measurement s of P-i release on the millisecond time scale with sensitivity in the 10 mu M range are obtained that provide new information about the rel ationship between ATP hydrolysis and force production.