CELL-ADHESION CONTROL BY ION-IMPLANTATION INTO POLYMERIC MATERIALS AND EXTRACELLULAR-MATRIX

The bio-compatibility of ion implanted polymers and extra-cellular mat rix has been studied by means of adhesion measurements of bovine aorta endothelial cells and the carcinoma of the cervix (HeLa cell). The sp ecimens used were polystyrene (PS), oxygen plasma treated polystyrene (PS-O), extra-cellular matrix (Collagen: Type I) coated polystyrene (P S-C), and gelatin coated polystyrene (PS-G). Ne+, Na+ and Ar+ ion impl antations were performed at energies of 50, 100 and 150 keV with fluen ce of 1 x 10(15) ions/cm(2) at room temperature. Ion implanted PS demo nstrated improvement of adhesion of endothelial cells and dramatic imp rovement of adhesion of HeLa cell. HeLa cell adhered only to ion impla nted circular domains of a diameter about 100 mu m on PS. By contrast, ion implanted PS-C, PS-G and PS-O domain inhibited HeLa cell adhesion . These phenomena were observed on Ne+, Na+ and Ar+ implanted specimen s at energies of 50, 100 and 150 keV. The results of cell adhesion to ion implanted PS was caused by carbon structure and new radicals induc ed by ion implantation. The inhibition of HeLa cell adhesion to ion im planted PS-C, PS-G and PS-O was caused by the destruction of cell adhe sion properties of amino acid, OH and > C=O by the radiation effects. A difference between endothelial and HeLa cell adhesion to the 150 keV -Ne+ ion implanted domain is recognized. It seems that 150 keV-Ne+ ion implantation with a fluence of 1 x 10(15) ions/cm(2) broke ligands on the collagen that corresponds to HeLa cell adhesive domain, and did n ot break ligands which corresponding to endothelial cell.