COVALENT COUPLING OF PROTEINS TO DIFFERENT STRAINS OF BACTERIA MODIFIED VIA SURFACE OXIDATION

Six different bacteria were investigated as protein carriers. Carbohyd rate structures of the cell wall were oxidised and human IgG, as a mod el protein, was bound covalently through Schiff base linkage. The resu lting bacteria - protein conjugates were characterised in terms of the amount of hlgG coupled per mass unit of bacteria. Agglutination react ions with sheep anti - hlgG antiserum illustrated the potential applic ation of this protein immobilised on bacteria as a solid support.