IMPROVEMENT OF THE THERMOSTABILITY OF BETA-GALACTOSIDASE FROM KLUYVEROMYCES-MARXIANUS

Comparative studies on some preparations of Kluyveromyces marxianus be ta-galactosidase show that enzyme localized in permeabilized cells and in glutaraldehyde treated permeabilized cells was more thermostable a t 45 degrees C, than cell-free enzyme, In contrast, the temperature op timum (50-52 degrees C) was not affected significantly by the intracel lular localization Permeabilized cells immobilized by entrapment into hardened calcium alginate gel or treated with glutaraldehyde were used for repeated lactose hydrolysis at 45 degrees C and showed good opera tional stability.