SPECIES DIAGNOSIS AND PHYLOGENY OF THE IPS GRANDICOLLIS GROUP (COLEOPTERA, SCOLYTIDAE) USING RANDOM AMPLIFIED POLYMORPHIC DNA

Bark beetle species of the Ips grandicollis group-I. hoppingi, I, conf usus, I. paraconfusus, I. montanus, I. grandicollis, I. cribricollis, and I. lecontei-are momphologically similar. Some of these species are sympatric and identification is problematic. We used the random ampli fied polylnorphic DNA (RAPE) technique, which yielded a diagnostic DNA banding pattern for each species. Blot hybridizations confirmed DNA s equence homology among comigrating DNA fragments. One most parsimoniou s phylogeny was reconstructed using bands as characters. Time phylogen y was supported by bootstrap values and congruent with a published phy logeny reconstructed from allozyme frequencies.