Ym. Chen et al., QUANTITATIVE-ANALYSIS OF MESSENGER-RIBONUCLEIC-ACID ENCODING NATRIURETIC PEPTIDE RECEPTORS IN ALDOSTERONE-PRODUCING ADENOMA, Molecular and cellular endocrinology, 111(2), 1995, pp. 139-146
There exist conflicting data regarding the inhibitory effect of atrial
natriuretic peptide on aldosterone production from aldosterone-produc
ing adenoma (APA). Natriuretic peptides mediate their actions through
natriuretic peptide receptors (NPRs). Whether or not NPRs are present
in the tumors remains controversial. To elucidate this paradox, gene e
xpression of NPRs was examined by Northern blot analysis and competiti
ve polymerase chain reaction in tumorous and non-tumorous portions of
APA, and in normal adrenal gland from patients with renal cell carcino
ma. The results of Northern blot analysis showed the presence of messe
nger ribonucleic acid (mRNA) of three NPRs in all adrenal tissues, inc
luding APA. The proportional expression of NPR gene transcripts in APA
was type A (0.6%), type B (18.7%), and type C (80.7%). The levels, bu
t not the proportions, of type C and possibly type B NPR mRNAs were lo
wer in tumorous and non-tumorous portions of APA compared to those in
normal adrenal gland (type C 190.2 +/- 24.5 [means +/- SEM, normal adr
enal gland] > 168.1 +/- 20.8 [non-tumorous portion] > 112.2 +/- 15.5 [
tumorous portion] pg/10 mu g total RNA, F = 3.82, P < 0.05; type B 45.
2 +/- 8.5 [normal adrenal gland] > 30.0 +/- 5.2 [non-tumorous portion]
> 25.1 +/- 4.1 [tumorous portion] pg/10 mu g total RNA, F = 3.03, P =
0.065). The mRNA levels of type C, rather than type A or type B, NPR
were correlated with the percentage of zona fasciculata-like cells in
APA (r = 0.90, P < 0.05). In conclusion we have demonstrated the prese
nce of mRNA encoding the three NPRs in APA, By quantitative analysis,
the proportional expression of the three subtypes is not different bet
ween APA and adrenal glands, with type C NPR being the most abundant,
followed by type B and type A. Type C and possibly type B NPR gene tra
nscripts were downregulated in both tumorous and non-tumorous portions
of APA. The gene expression of GC-coupled NPRs of APA is not histolog
ically dependent.