Pre-incubation of human sperm in egg yolk based medium (TEST-yolk) as
well as milk have been shown to improve sperm fertilizing capacity. Th
e purpose of this study was to compare a combination of these two medi
a, called milk-yolk, against the two established media, in order to te
st for a possible synergistic effect(s) on sperm functions as measured
by sperm motility, acrosome reaction and penetration assay. Statistic
ally, no differences have been observed among the three media, except
for SPA outcome in milk-yolk, being significantly lower than the corre
sponding values for the other two media. In conclusion the beneficial
effects of egg yolk and milk appear to be eliminated once both media a
re combined, resulting in an antagonistic effect on the fertilizing ca
pacity of the spermatozoa.