AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY (ELISA) FOR DETECTION OF SEMINALFLUID USING A MONOCLONAL-ANTIBODY TO PROSTATIC ACID-PHOSPHATASE

Authors
ALLEN SM
Citation
Sm. Allen, AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY (ELISA) FOR DETECTION OF SEMINALFLUID USING A MONOCLONAL-ANTIBODY TO PROSTATIC ACID-PHOSPHATASE, Journal of immunoassay, 16(3), 1995, pp. 297-308
Citations number
19
Categorie Soggetti
Immunology
Journal title
Journal of immunoassay → ACNP
ISSN journal
01971522
Volume
16
Issue
3
Year of publication
1995
Pages
297 - 308
Database
ISI
SICI code
0197-1522(1995)16:3<297:AE(FDO>2.0.ZU;2-V
Abstract
A microtitre plate format enzyme linked immunosorbent assay (ELISA), e mploying commercially available PASE/4LJ mouse monoclonal hybridoma an tibody is described. The technique is a solid phase indirect ELISA for prostatic acid phosphatase, applicable to specific detection of semen . Maximal detectability was found to be one hundred thousand fold dilu tion of pooled seminal plasma. No cross reactivities with human vagina l fluid, blood, saliva, female urine, nasal discharge, earwax, sweat o r faeces have been found.