GLUCOSINOLATE BIOSYNTHESIS IN OILSEED RAPE (BRASSICA-NAPUS L) - STUDIES WITH (SO42-)-S-35 AND GLUCOSINOLATE PRECURSORS USING OILSEED RAPE PODS AND SEEDS
D. Toroser et al., GLUCOSINOLATE BIOSYNTHESIS IN OILSEED RAPE (BRASSICA-NAPUS L) - STUDIES WITH (SO42-)-S-35 AND GLUCOSINOLATE PRECURSORS USING OILSEED RAPE PODS AND SEEDS, Journal of Experimental Botany, 46(288), 1995, pp. 787-794
(SO42-)-S-35 and glucosinolate precursors were administered to investi
gate the capacity of oilseed rape intact whole pods, isolated pod wall
s and seeds to carry out reactions of the glucosinolate biosynthetic p
athway, Pod walls incorporated S-35-label from (SO42-)-S-35 into both
the thio-glucose and sulphonate-sulphur of glucosinolates, Pulse-label
ling experiments showed that pod walls were the predominant source of
the glucosinolates accumulated by the seeds. Isolated immature seeds w
ere capable of reductive assimilation of (SO42-)-S-35 and incorporated
S-35 into sulphur-containing amino acids and the sulphonate moiety of
glucosinolates, but were not able to incorporate S-35 into the thio-g
lucose of glucosinolates.