BIOCHEMICAL-PROPERTIES AND FUNCTION OF POLY(ADP-RIBOSE) GLYCOHYDROLASE

We describe here the latest observations on poly(ADP-ribose) glycohydr olase. There is now extensive evidence that this nuclear enzyme is an endo-exoglycosidase which has a key role to perform in the removal of polymers which interact with proteins through covalent and non-covalen t interactions. Also, we have developed a zymogram which will permit t he isolation of the various isoforms of the glycohydrolase and the eve ntual cloning of this enzyme. Finally, we have evidence that very shor t oligomers and even monomers of ADP-ribose covalently bound to protei ns can be removed by poly(ADP-ribose) glycohydrolase.