SEX STEROIDS UP-REGULATE E-CADHERIN EXPRESSION IN HORMONE-RESPONSIVE LNCAP HUMAN PROSTATE-CANCER CELLS

There is convincing evidence that a reduced expression of the E-cadher in cell-cell adhesion molecule associates with low tumor grade and poo r prognosis in prostate cancer patients. However, little is known on h ow E-cadherin levels are regulated in human prostate cancer cells, We have inspected the effect of both androgens and estrogen on the expres sion of E-cadherin in the hormone-responsive LNCaP prostate tumor cell line, which is endowed with both androgen and estrogen receptors, Usi ng both Dot Blot analysis and immunocytochemistry we have observed tha t either steroid significantly increased E-cadherin levels in these ce lls; this effect was not reversed by the simultaneous addition of the relevant antagonist, hydroxyflutamide or ICI-182,780. (C) 1995 Academi c Press. Inc.