LOSS OF HETEROZYGOSITY IN CHROMOSOME-1, CHROMOSOME-5, CHROMOSOME-7 AND CHROMOSOME-13 IN MOUSE HEPATOMA DETECTED BY SYSTEMATIC GENOME-WIDE SCANNING USING RLGS GENETIC-MAP

Authors
OHSUMI T OKAZAKI Y OKUIZUMI H SHIBATA K HANAMI T MIZUNO Y TAKAHARA T SASAKI N UEDA M MURAMATSU M KERNS KA CHAPMAN VM HELD WA HAYASHIZAKI Y
Citation
T. Ohsumi et al., LOSS OF HETEROZYGOSITY IN CHROMOSOME-1, CHROMOSOME-5, CHROMOSOME-7 AND CHROMOSOME-13 IN MOUSE HEPATOMA DETECTED BY SYSTEMATIC GENOME-WIDE SCANNING USING RLGS GENETIC-MAP, Biochemical and biophysical research communications, 212(2), 1995, pp. 632-639
Citations number
21
Categorie Soggetti
Biology,Biophysics
Journal title
Biochemical and biophysical research communications → ACNP
ISSN journal
0006291X
Volume
212
Issue
2
Year of publication
1995
Pages
632 - 639
Database
ISI
SICI code
0006-291X(1995)212:2<632:LOHICC>2.0.ZU;2-K
Abstract
We have developed an RLGS-based scanning system to detect DNA alterati on in tumor tissues, using 575 mapped spots/loci in a single gel. This system is very powerful for screening and identifying not only loss o f heterozygosity (LOH) but also DNA methylation change. In this study, we applied this system to search for the LOH of hepatoma from an inte rspecific F-1 hybrid between Mus spretus and C57BL/6 with SV40 early T antigen transgene connected to a mouse major urinary protein enhancer /promoter. Comparing the RLGS profiles of each tumor to that of the no rmal tissue showed significant LOH in chromosomes 1,5,7 and 13. (C) 19 95 Academic Press, Inc.