CLONING AND CHROMOSOMAL LOCALIZATION OF THE HUMAN ILEAL LIPID-BINDINGPROTEIN

A human ileal lipid-binding protein (ILBP(3)) cDNA was isolated and sh own to encode a 128 amino acid protein with homology to the fatty acid -binding protein gene family. The human ILBP amino acid sequence exhib ited 78% identity to the rat ILBP sequence. Northern blot analysis rev ealed a single transcript of approx. 0.6 kb in small intestine, but no hybridization to liver, spleen, thymus, prostate, ovary, caecum, or c olon. Southern blot analysis of genomic DNA from a panel of rodent-hum an somatic cell hybrids revealed that the human ILBP gene resides on c hromosome 5 and is not linked to any other known fatty acid-binding pr otein family member.