GENOTYPING APOLIPOPROTEIN-B SIGNAL PEPTIDE INSERTION DELETION - A COMPARISON OF 3 METHODS

Authors
GLISIC S SUNJEVARIC I ALAVANTIC D
Citation
S. Glisic et al., GENOTYPING APOLIPOPROTEIN-B SIGNAL PEPTIDE INSERTION DELETION - A COMPARISON OF 3 METHODS, Electrophoresis, 16(6), 1995, pp. 899-902
Citations number
14
Categorie Soggetti
Biochemical Research Methods
Journal title
Electrophoresis → ACNP
ISSN journal
01730835
Volume
16
Issue
6
Year of publication
1995
Pages
899 - 902
Database
ISI
SICI code
0173-0835(1995)16:6<899:GASPID>2.0.ZU;2-3
Abstract
Three different ways of detecting insertion/deletion (Ins/Del) genotyp es of the apolipoprotein B gene encoding signal peptide were compared. Unequal quantities of the two alleles were observed in 50 Serbian sub jects. The low abundance allele was clearly visible only when a modifi ed method of silver staining for polyacrylamide gels was used. This me thod is more reliable for Ins/Del polymorphism genotyping, avoiding mi sclassifications by conventional ethidium bromide staining of both aga rose and polyacrylamide gels.