ENHANCED KARYOTYPE RESOLUTION OF CRYPTOSPORIDIUM-PARVUM BY CONTOUR-CLAMPED HOMOGENEOUS ELECTRIC-FIELDS

Authors
HAYS MP MOSIER DA OBERST RD
Citation
Mp. Hays et al., ENHANCED KARYOTYPE RESOLUTION OF CRYPTOSPORIDIUM-PARVUM BY CONTOUR-CLAMPED HOMOGENEOUS ELECTRIC-FIELDS, Veterinary parasitology, 58(4), 1995, pp. 273-280
Citations number
19
Categorie Soggetti
Parasitiology,"Veterinary Sciences
Journal title
Veterinary parasitology → ACNP
ISSN journal
03044017
Volume
58
Issue
4
Year of publication
1995
Pages
273 - 280
Database
ISI
SICI code
0304-4017(1995)58:4<273:EKROCB>2.0.ZU;2-W
Abstract
Previous karyotype analysis with contour-clamped homogeneous electric fields (CHEF) indicated that the Cryptosporidium parvum karyotype comp rises a minimum of five chromosomes all less than 1.6 Mb in size. In t his study we were able to improve the resolution of the karyotype usin g a modified CHEF gel electrophoretic procedure that allows for the ro utine identification of seven C. paurvum chromosomes ranging in size f rom about 0.945 to 2.2 Mb. Our data also suggest that an eighth chromo some is present and comigrates with another chromosome at about 1.3 Mb .