THE COMPLETE H-1-NMR ASSIGNMENTS OF AMINOGLYCOSIDE ANTIBIOTICS AND CONFORMATIONAL STUDIES OF BUTIROSIN-A THROUGH THE USE OF 2D NMR-SPECTROSCOPY

The complete proton assignments of the aminoglycoside antibiotics, but irosin A, kanamycin A and kanamycin B, at pH 6.5 have been made throug h the use of various homonuclear and heteronuclear 2D NMR methods. But irosin A NOESY experiments suggest a stacking arrangement between the xylose and 2,6-diamino-2,6-dideoxyglucose rings, while the 2-deoxystre ptamine ring and its substituent, the (S)-4-amino-2-hydroxybutyryl gro up, extend away from the stacked rings. informative long-range NOEs we re observed for butirosin A but not with kanamycin A or kanamycin B. M any intra-ring NOEs were observed with all three aminoglycosides that confirm the proton assignments made in this study.